Biological activity of dried leaves from Neem tree, Azadirachta indica, on the Aedes aegypti larvae
Abstract
This paper aimed to produce experimental evidences on the possibility of the use the dried leaf of the neem tree, Azadirachta indica, aqueous extract as a natural insecticide to Aedes aegypti, the vector of Dengue. It is justified by the fact that the available wide range insecticides exterminate indiscriminately dangerous as well as useful insects to mankind. Another problem of wide range insecticides is the growing resistance shown by insects to them, what had been leading to the demands of higher concentrations at each application. The larval mortality of A. aegypti at the 3rd and 4th stages exposed to extract concentrations of 0.4% and 0.8% suggest a consistent perspective for its use as insecticide, when compared to the mortality of non-treated ones. The bioassay had shown wide possibilities to the use of dried leaves extract of neem tree as a natural insecticide to control dengue. In inspite of that, some other trials should be done to consolidate the methodology of water instead of organic solvent extraction of dried leaves of A. indica in the natural control of A. aegypti.
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References
Batra CP, Mittal PK, ADAK T & Sharma VP (1998) Efficacy of neem oil-water emulsion against mosquito immatures. Indian Journal of Malariology 35: 15-21.
Boschitz C & Grunewald J (1994) The effect of NeemAzal on Aedes aegypti (Diptera: Culicidae). Applied Parasitology 35: 251-256.
El-Shazly MM & El-Sharnoubi ED (2000) Toxicity of a neem (Azadirachta indica) insecticide aquatic organisms. Journal of the Egyptian Society of Parasitology 30: 221-231.
Falesi IC, Ferreira CAP & Carvalho R (2000) Análise econômica da produção do nim indiano no estado do Pará. Comunicado Técnico. Belém: EMBRAPA, Amazônia Oriental, 43: 4.
Hedge NG (1993) Improving the producivity of neem trees. World Neem Conference, Indian Journal of Entomology 50: 147-150.
Hosmer DW & Lemeshow S (1989) Applied logistic regression. New York: John Wiley.
Jones PS, Ley SV, Morgan ED & Santafianos D (1989) The chemistry of the neem tree. In: Jacobson M (ed.) Focus on phytochemical pesticides, the neem tree. Florida: CRC, 1: 47-67.
Kraus W (1995) Biologically active ingredients. In: Schmutteres H (ed.), The neem tree: source of unique natural products for integrated pest management, medicine, industry and other purposes. Weinheim: VCH, pp.35-88.
Lee SM, Klocke JA, Barnby MA, Yasmasaki RB & Balandrin MF (1991). Insecticidal constituents of Azadirachta indica and Melia azedarach (Meliaceae). ACS Symposium Series 449: 293-304.
Martinez SS & van Emden HF (2001) Growth disruption, abnormalities and mortality of Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuidae) caused by azadirachtin. Neotropical Entomology 30: 113-125.
Martinez SS (2002) O Nin – Azadirachta indica: natureza, usos múltiplos, produção. Londrina: Instituto Agronômico do Paraná.
Mitchell MJ, Smith SL, Johnson S & Morgan ED (1997) Effects of the neem tree compounds azadirachtin, salannin, nimbin, and 6-desacetylnimbin on ecdysone 20-monooxygenase activity. Archives of Insect Biochemistry and Physiology 35: 199-209.
Mordue (Luntz) AJ, Cottee PK & Evans KA (1985) Azadirachtin: its effect on gut motility, growth and moulting in Locusta. Physiological Entomology 10: 431-437.
Nagpal BN, Srivastava A & Sharma VP (1995) Control of mosquito breeding using wood scrapings treated with neem oil. Indian Journal of Malariology 32 64-69.
Rembold H (1995) Biological effect of neem and their modes of action. Growth and metamorphosis. In Schmutteres H (ed) The Neem tree: source of unique natural products for integrated pest management, medicine, industry and other purposes. Weinheim: VCH, pp.177-194.
Salehzadeh A, Jabbar A, Jennens L, Ley SV, Annadurai RS, Adams R Strang RH (2002) The effects of phytochemical pesticides on the growth of cultured invertebrate and vertebrate cells. Pest Management Science 58: 268-276.
Schneider B & Ermel K (1987).Quantitative determination of azadirachtin from neem seeds using high performace liquid chromatography. In: Scmutterer H & Ascher KRS (eds) Natural pesticides from the neem tree and other tropical plants. Proceedings of the 3 International Neem Conference. Nairobi: Eschborn, pp. 161-170.
Siddiqui BS, Afshan F, Ghiasuddin-Faizi S, Naqvi SN & Tariq RM (2000) Two insecticidal tetranortriterpenoids from Azadirachta indica. Phytochemistry 53: 371-376.
Sivagnaname N & Kalyanasundaram M (2004). Laboratory evaluation of methanolic extract of Atlantia monophylla (Family: Rutaceae) against immature stages of mosquitoes and non-target organisms. Memórias do Instituto Oswaldo Cruz 99: 115-118.
Wandscheer CB, Duque JE, Silva MA, Fukuyama Y, Wohlke JL, Adelmann J & Fontana JD (2004) Larvicidal action of ethanolic extracts from fruit endocarps of Melia azedarach and Azadirachta indica against the dengue mosquito Aedes aegypti. Toxicon 44: 829-835.
Warthen Jr JD (1989) Neem: organisms affected and reference list update. Proceeding of the Entomological Society of Washington 91: 367-388.
Wigglesworth VB (1972) The principles of insect physiology. 7 ed. New York: John Wiley.
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