Desvendando a H+-Pirofosfatase vacuolar e o seu papel na biotecnologia vegetal
Resumen
As proteínas de membrana são de fundamental importância para manter o funcionamento da célula e a homeostase iônica dos seres-vivos. Há uma diversidade de proteínas que ajudam a manter o gradiente iônico entre o meio intra e extracelular, entre as quais, a H+-ATPase e a H+-pirofosfatase (H+-PPase) tem um importante destaque. A H+-PPase está presente na membrana do vacúolo das células das plantas e sua principal função é transportar H+ para o lúmem, criando uma força elétron-motriz que impulsiona o transporte secundário de íons e compostos orgânicos. Esta enzima é diferencialmente regulada em condições de stress conferindo uma maior tolerância das plantas aos fatores bióticos e abióticos. Atualmente, sabe-se que o gene que codifica a H+-PPase quando super-expressado, estimula o crescimento vegetal via um maior desenvolvimento radicular e, conseqüentemente, maior absorção de água e nutrientes. Além disso, uma maior resistência ao stress salino foi observada. Novos estudos são realizados para elucidar os processos fisiológicos causados pela super-expressão da H+-PPase objetivando uma possível aplicação na produção vegetal, em solos com baixa fertilidade.
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Baykov AA, Bakuleva NP & Rea PA (1993) Steady-state kinetics of substrate hydrolysis by vacuolar H+-pyrophosphatase: a simple three- state model. European Journal of Bichemstry 217: 755-762.
Baykov AA, Cooperman BS, Goldman A & Lahti R (1999) Cytoplasmic inorganic pyrophosphatase. Progress in Molecular and Subcellular Biolology 23: 127-150.
Britten CJ, Turner JC & Rea PA (1989) Identification and purification of substrate-binding subunit of higher plant H+-translocating inorganic pyrophosphatase. FEBS Letters 256: 200-206.
Canellas LP, Olivares FL, Okorokova-Façanha AL & Façanha AR (2002) Humic Acids Isolated from Earthworm Compost Enhance Root Elongation, Lateral Root Emergence, and Plasma Membrane H+ATPase Activity in Maize Roots. Plant Physiology 130: 1951–1957.
Carystinos GD, MacDonald HR, Monroy AF, Dhindsa RS & Poole RJ (1995) Vacuolar H+-translocating pyrophosphatase is induced by anoxia or chilling in seedlings of rice. Plant Physiology 108: 641– 649.
Cooperman BS, Baykov AA& Lahti R(1992) Evolutionaryconservation of the active site of soluble inorganic pyrophosphatase. Trends in Biochemical Sciences 17: 262-266.
Davies JM, Darley CP & Sanders D (1997) Energetics of the plasma membrane pyrophosphatase. Trends in Plant Sciences 2: 9–10.
Davies JM, Poole RJ, Rea PA & Sanders D (1992) Potassium transport into plant vacuoles energized directly by a proton- pumping inorganic pyrophosphatase. Proceedings of the National Acaddemy of Sciences USA 89: 11701-11705.
Davies JM, Poole RJ & Sanders D (1993) Analysis of the substrate binding site and carboxyl terminal region of vacuolar H+- pyrophosphatase of mung bean with peptide antibodies. Biochimica et Biophysica Acta 1141: 29-36.
Drozdowicz YM & Rea PA (2001) Vacuolar H+-pyrophosphatases: from evolutionary backwaters into mainstream. Trends in Plant Science 6: 206–211.
Drozdowicz YM, Lu YP, Patel V, Fitz-Gibbon S, Miller JH & Rea RA (1999) A thermostable vacuolar-type membrane pyrophosphatase from the archaeon Pyrobaculum aerophilum: implications for the origins of pyrophosphate-energized pumps. FEBS Letters 460: 505-512.
Façanha AR & Méis L (1998) Reversibility of H1-ATPase and H1- Pyrophosphatase in Tonoplast Vesicles from Maize Coleoptiles and Seeds. Plant Physiology 116: 1487–1495.
Façanha AR, Façanha ALO, Olivares FL, Guridi F, Santos GA, Velloso ACX, Rumjanek VM, Brasil F, Schripsema J, Braz-Filho R, Oliveira MA & Canellas LP (2002) Bioatividade de ácidos húmicos: efeitos sobre o desenvolvimento radicular e sobre a bomba de prótons da membrana plasmática. Pesquisa Agropecuária Brasileira 37: 1301-1310
Ferrol N, Pozo MJ, Antelo M & Azcón-Aguilar C (2002) Arbuscular mycorrhizal symbiosis regulates plasma membrane H+-ATPase gene expression in tomato plants. Journal of Experimental Botany 53: 1683–1687.
Gaxiola RA, Fink GR& Hirschi KD(2002) Geneticmanipulationofvacuolar proton pumps and transporters. Plant Physiology 129: 967-973.
Gaxiola RA, Palmgren MG & Schumacher K (2007) Plant proton pumps. FEBS Letters 581: 2204-2214.
Gaxiola RA, Rao R, Sherman A, Grisafi P, Alper SL & Fink GR (1999) The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast. Proceedings of the National Acaddemy of Sciences USA 96: 1480-1485.
Gianinazzi-Pearson V, Arnould C, Oufattole M, Arango M & Gianinazzi S (2000) Differential activation of H+-ATPase genes by an arbuscular mycorrhizal fungus in root cells of transgenic tobacco. Planta 211: 609–613.
Gordon-Weeks R, Steele SH & Leigh RA (1996) The role of magnesium, pyrophosphate, and their complexes as substrates and activators of the vacuolar H+-pumping inorganic pyrophosphatase (studies using ligand protection from covalent inhibitors). Plant Physiology 111: 195-202.
Jahn T, Fuglsang AT, Olosson A, Brüntrup IM, Collinge DB, Volkmann D, Sommarin M, Palmgren MG & Larsson C (1997) The 14-3-3 protein interacts directly with the c-terminal region of the plant plasma membrane H+-ATPase. The Plant Cell 9: 1805-1814.
Kasai M, Nakamura N, Kudo N, Sato H, Maeshima M & Sawada S (1998) The activity of the root vacuolar H+-pyrophosphatase in rye plants grown under conditions deficient in mineral nutrients. Plant Cell Physiology 39: 890-894.
Kim Y, Kim EJ & Rea PA (1994) Isolation and characterization of cDNAs encoding the vacuolar H+-pyrophosphatase of Beta vulgaris. Plant Physiology 106: 375-382.
Krajinski F, Hause B, Gianinazzi-Pearson V & Franken P (2002) Mtha1, a plasma membrane H+-ATPase gene from Medicago truncatula, shows arbuscule-specific induced expression in mycorrhizal tissue. Plant Biology 4: 754–761.
Leigh RA, Pope AJ, Jennings IR & Sanders D (1992) Kinetics of the vacuolar H+-pyrophosphatase from higher plants. The role of magnesium, pyrophosphate and their complexes as substrates, activators and inhibitors. Plant Physiology 100: 1698-1705.
Lerchl J, König S, Zrenner R & Sonnewald U (1995) Molecular cloning, characterization and expression analysis of isoforms encoding tonoplast-bound proton-translocating inorganic pyrophosphatase in tobacco. Plant Molecular Biology 29: 833-840.
Li J, Yang H, Peer WA, Richter G, Blakeslee J, Bandyopadyay A, Titapiwantakun B, Undurraga S, Khodakovskaya M, Richards EL, Krizek B, Murphy AS, Gilroy S & Gaxiola R (2005) Arabidopsis H+-PPase AVP1 regulates auxin-mediated organ development. Science 310: 121-125.
Long AR, Williams LE, Nelson SJ & Hall JL (1995) Localization of membrane pyrophosphatase activity in Ricinus communis seedlings. Journal of Plant Physiology 146: 629-638.
Maeshima M & Yoshida S (1989) Purification and properties of vacuolar membrane proton-translocating inorganic pyrophosphatase from mung bean. Journal of Biological Chemistry 264: 20068-20073.
Maeshima M (1990) Development of vacuolar membranes during elongation of cells in mung bean hypocotyls. Plant Cell Physiology 31: 311-317.
Maeshima M (1991) H(+)-translocating inorganic pyrophosphatase of plant vacuoles. Inhibition by Ca2+, stabilization by Mg2+ and immunological comparison with other inorganic pyrophosphatases. European Journal of Biochemical 196: 11-17.
Maeshima M (2000) vacuolar H+-pyrophosphatase. Biochimica et Biophysica Acta 1465: 37-51.
Maruyama C, Tanaka Y, Mitsuda NT, Takeyasu K, Yoshida M & Sato MH (1998) Structural studies of the vacuolar H+- pyrophosphatase: sequence analysis and identification of the residues modified by fluorescent cyclohexylcarbodiimide and maleimide. Plant Cell Physiology 39: 1045-1053.
Moriau L, Michelet B, Bogaerts P, Lmabert L, Michel A, Oufattole M & Boutry M (1999) Expression analysis of two gene subfamilies encoding the plasma membrane H+-ATPase in Nicotiana plumbaginifolia reveals the major transport functions of this enzyme. The Plant Journal 19: 31-41.
Morsomme P & Boutry M (1999) The plant mebrana H+- ATPase: structure, function and regulation. Biochimica et Biophysica Acta 1465: 1-16.
Murphy PJ, Langridge P, Smith SE (1996) Cloning plant genes differentially expressed during colonization of roots of Hordeum vulgare by the vesicular–arbuscular mycorrhizal fungus Glomus intraradices. New Phytologist 135: 291–301.
Nakanishi Y & Maeshima M (1998) Molecular cloning of vacuolar H+- pyrophosphatase and its developmental expression in growing hypocotyl of Mung Bean. Plant Phisiology 116: 598-597.
Nore BF, Sakai-Nore Y, Maeshima M, Baltscheffsky M & Nyrén P (1991) Immunological cross-reactivity between proton- pumping inorganic pyrophosphatases of widely phylogenic separated species. Biochemical and Biophysical Research Communications 181: 962-967.
Obermeyer G, Sommer A & Bentrup F-W (1996) Potassium and voltage dependence of the inorganic pyrophosphatase of intact vacuoles from Chenopodium rubrum. Biochimica et Biophysica Acta 1284: 203-212.
Palmgren MG, Sommarin M, Serrano R & Larsson C (1991) Identification of an autoinhibitory domain in the c-terminal region of the plant plasma membrane H+-ATPase. Journal of Biological Chemistry 266: 20470-20475.
Park S, Li J, Pittman JK, Berkowitz GA, Yang H, Undurraga S, Morris J, Hirschi KD & Gaxiola RA (2005) Up-regulation of H+-pyrophosphatase (H+-Ppase) as a strategy to engineer drought-resistant crop plants. Proceedings of the National Acaddemy of Sciences USA 102: 18830-18835.
Pérez-Castiñeira JR, López-Marquéz RL, Villalba JM, Losada M & Serrano A (2002) Functional complementation of yeast cytosolic phyrophosphatase by bacterial and plant H+-translocating pyrophosphatases. Proceedings of the National Acaddemy of Sciences USA 99: 15914-15919.
Ramos AC, Martins MA & Façanha RA (2005) Atividade atpásica e pirofosfatásica em microcromossomos de raízes de milho colonizadas com fungos micorrízicos arbusculares. Revista Brasileira de Ciência do Solo 29: 207-213
Rea PA & Poole RJ (1985) Proton-translocating inorganic pyrophosphatase in Red Beet (Beta vulgaris L.) Tonoplast Vesicles. Plant Physiology 77: 46-52
Rea PA & Poole RJ. (2000) Vacuolar H+-translocating pyrophosphatase Annual Review of Plant Physiology and Plant Molecular Biology 44: 157-180.
Rea PA, Kim Y, Sarafian V, Poole RJ, Davies JM & Sanders D (1992) Vacuolar H+-translocating pyrophosphatases: a new category of ion translocase. Trends in Biochemical Sciences 17: 348-353.
Robinson DG, Hoppenrath M, Oberbeck K, Luykx P & Ratajczak R (1998) Localization of pyrophosphatase and V-ATPase in Chlamydomonas reinhardtii. Botanica Acta 111: 108-122.
Sakakibara Y, Kobayashi H & Kasamo K (1996) Solation and characterization of cDNAs encoding vacuolar H+- pyrophosphatase isoforms from rice (Oryza sativa L.). Plant Molecular Biology 31: 1029-1038.
Sarafian V & Poole RJ (1989) Purification of an H+-translocating inorganic pyrophosphatase from vacuole membranes of Red Beet. Plant Physiology 91: 34-38.
Sarafian V, Kim Y, Poole RJ & Rea PA (1992) Molecular cloning and sequence of cDNA encoding the pyrophosphate-energized vacuolar membrane proton pump of Arabidopsis thaliana. Proceedings of the Natural Academy Sciences USA 89: 1775-1779.
Sato MH, Kasahara M, Ishii N, Homareda H, Matsui H, Yoshida M (1994) Purified vacuolar inorganic pyrophosphatase consisting of a 75-kDa polypeptide can pump H+ into recontituted proteoliposomes. Journal of Biological Chemistry 269: 6725-6758.
Scott DA, Souza W de, Benchimol M, Zhong L, Lu HG, Moreno SNJ & Docampo R (1998) Presence of a plant-like proton pumping pyrophosphatase in acidocalcisomes of Trypanosoma cruzi. Journal of Biological Chemistry 273: 22151-22158.
Sze H, Li X & Palmgren MG (1999) Energization of plant cell membranes by H+-pumping ATPases: regulation and biosynthesis. The Plant Cell 11: 677-689.
Taiz L & Zeiger E (2002) Plant Physiology. 3ed. Sinauer.
Taiz L (1992) The plant vacuole. Journal of Experimental Biology 172: 113-122.
Takasu A, Nakanishi Y, Yamauchi T & Maeshima M (1997) Analysis of the substrate binding site and carboxyl terminal region of vacuolar H+-pyrophosphatase of Mung Bean with peptide antibodies Journal of Biochemistry 122: 883-889.
Tanaka Y, Chiba K, Maeda M & Maeshima M (1993) Molecular cloning of cDNA for vacuolar membrane proton-translocating inorganic pyrophosphatase in Hordeum vulgare. Biochemistry and Biophysics Research Communication 190: 1110-1114.
Zandonadi DB, Canellas LP & Façanha AR (2007) Indolacetic and humic acids induce lateral root development through a concerted plasmalemma and tonoplast H+ pumps Planta 225:1583-1595.
Zhen RG, Baykov AA, Bakuleva NP & Rea PA (1994) Aminomethylenediphosphonate: a potent type-specific inhibitor of both plant and phototrophic bacterial H+- pyrophosphatases. Plant Physiology 104: 153-159.
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