Chlorhexidine Gluconate in preventing Surgical Site Infection
Abstract
The surgical site infection (SSI) is a complication that affects exposed tissues during surgery. Skin preparation, during antisepsis, reduces the chances of SSI. Antisepsis is the chemical disinfection of living tissues, based on the application of microbicidal agentes. One of the most used antiseptics is Chlorhexidine Gluconate presented in alcoholic, aqueous or degerming solution. The study aimed to evaluate the efficiency of the antiseptic Chlorhexidine Gluconate in degerming solution associated or not with Chlorhexidine Gluconate in alcoholic solution, in preventing SSI. The research, approved by CEUA/ESFA under protocol 009/2018, was conducted at the Veterinary School Clinic Dr. Laurindo Costa Neto of ESFA between August and December 2018. The 24 female dogs selected were subjected to ovariohysterectomy whereas twelve of them were allocated to Group 1 (G1), in which Chlorhexidine Gluconate was used in a degerming solution. The other twelve female dogs were allocated to Group 2 (G2), in which Chlorhexidine Gluconate in degerming solution was used, followed by Chlorhexidine Gluconate in alcoholic solution. Sterile skin swabs from the surgical site were collected in three moments: after trichotomy (T1), after antisepsis (T2) and at the end of surgery (T3). After that, the samples were inoculated in Tryptone Soya Broth (TSB) medium and seeded on blood agar. The results obtained showed that there were microbial growth in T1 for all samples. There was no growth in T2 for all samples either. In T3, there was bacterial growth in one G1 sample and in three G2 samples. Statistically, through the chi-square test (X²), the results obtained did not show differences in the quality of antisepsis and, in the conditions and characteristics of this study, both G1 and G2 were eficiente. Hence, the antiseptic Chlorhexidine Gluconate in a degerming solution, even without associated with Chlorhexidine Gluconate in alcoholic solution, was efficient in preventing the SSI of female dogs submitted to ovary-hysterectomy.
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