Evaluation of the mussel Perna perna (Linnaeus, 1758) celular response in Espírito Santo Bay by neutral red retention time (NRRT)
Abstract
The biomarkers analysis in low tropic levels it’s so important because this is an alert in high tropic levels previously the impact be noted for conventional studies or by the population. The investigation of Espírito Santo Bay condition was realized with the bivalve Perna perna ( Linnaeus, 1758) by Neutral Red Retention Time (NRRT). Was analyzed mussels of three different points of Espírito Santo Bay (Boi Island, Frade Island and Third Bridge), and one reference point in Setiba, Guarapari –ES. This study reveal that the contamination in this points (save Setiba) originate oxidative damage that resulted in lisossomal damage. The lower mean time of retention was observable in mussels population of Third Bridge (35,25 minutes), followed by Boi Island (54 minutes) and Frade Island (70,5 minutes) in relation of the reference point (115,5 minutes). Had statically significant between in the mean times of retention points considerate polluted in relation of reference point. Retention times inferior a 60 minutes reveal severe damage in the lisossomal compartment, propose contamination of Third Bridge and Boi Island.
Keywords:
Downloads
References
Barnes RD (2006) Zoologia dos Invertebrados. 7 ed. São Paulo: Roca (SP).
Borenfreund E, Puerner JA (1985) Toxicity determinate in vitro by morphological alterations and neutral red absorption. Toxicolog y Letters 24: 119-124.
Cheung VV, Wedderburnm RJ, Depledge MH (1998) Molluscan lysosomal responses as diagnostic tool for the detection of a pollution gradient in Tolo Harbour, Hong Kong. Marine Environmental Research 46: 237-241.
Depledge MH, Fossi MC (1994) The role of biomarkers in environmental assessment (2). Invertebrates Ecotoxicolog y 3: 161-172.
Fossi MC, Casini S, Savelli C, Corbelli C, Franchi E, Mattei N.; Sanchez-Hernandez JC, Corsi I, Bamber S, Depledge MH (2000) Biomarker responses at different levels of biological organization in crabs (Carcinus aestuarii) experimentally exposed to benzo[a]pyrene. Chemosphere 40: 861-874.
Francioni E, Wagener ALR, Cavalier B, Scofield AL (2005) Biomonitoring of polycyclic Aromatic hydrocarbon in Perna perna from Guanabara Bay, Brazil. Environmental Forensics 6: 361-370.
Francioni E, Wagener ALR, Scofield AL, Depledge MH, Cavalier B (2007). Evaluation of the mussel Perna perna as a biomonitor of polycyclic aromatic hydrocarbon (PAH) exposure and effects. Marine Pollution Bulletin 54: 329-338.
Furley TH (1993) Utilização do mexilhão Perna perna (Linné, 1758) como bioindicador dos metais pesados cádmio, chumbo, zinco, cobre e manganês do litoral do Rio Grande do Sul (Brasil). Dissertação de Mestrado. Porto Alegre: Fundação Universidade do Rio Grande.
Gaze FN (2005) Tempo de residência na Baía do Espírito Santo. Monografia. Vitória: Universidade Federal do Espírito Santo.
Huggett R, Limerle RA, Mehrle PM, Bergman HL (1992) Biomarkers: biochemical, physiological and histological markers of anthropogenic stress. Chelsea: Lewis Publishers.
Jesus HC, Costa EA, Mendonça ASF, Zandonade E (2004) Distribuição de metais pesados em sedimentos do sistema estuarino da Ilha de Vitória- ES. Química Nova 27: 378-386.
Krohling W, Freitas Netto R, Brum SM (2004) Levantamento preliminar da estrutura da comunidade bentônica da zona entremarés da Ilha do Frade, Vitória - ES. In: XXV Congresso Brasileiro de Zoologia. Brasília. Sociedade Brasileira de Zoologia.
Lowe DM, Fossato VU, Depledge MH (1995) Contaminant-induced lysossomal membrane damage in blood cells of mussel Mytillus galloprovinciales from the Venice Lagoon: in vitro study. Marine Ecolog y Progress Series 129: 189-196.
Lowe DM, Pipe RK (1992) Contaminant induced lysossomal menbrane damage in marine mussel digestive cell: in vitro study. Aquatic Toxicology 30: 357-365.
McCarthy JF, Shugart LR (1990) Biological Markers of Environmental Contamination. In: McCarthy JF, Shugart, LR (ed). Biomarkers of Environmental Contamination. Chelsea: Lewis Publishers, p. 3-14.
Nascimento IA, Sousa ECPM, Nipper M (2002). Métodos em ecotoxicologia marinha; aplicações no Brasil. Rio de Janeiro: Artes Gráficas e Indústria Ltda.
Pereira CDS, Abessa DMS, Zaroni LP, Gasparro MR, Bícego M C, Taniguchi S, Furley TH,Sousa ECPM (2007) Integrated assessment of multilevel biomarker responses and chemical analysis in mussels from São Sebastião, São Paulo, Brazil. Environmental Toxicolog y and Chemistry 26: 462-469.
Santos MC (2004) Morfometria de Tivela mactroides (Born,1778) (bivalvia: veneridae) da Praia de Camburi, Vitória, Espírito Santo. Monografia de Graduação: Santa Teresa: Escola Superior São Francisco de Assis.
Souza EM (2002) Avaliação da contaminação por metais pesados (Cd, Cr, Cu, Fe, Ni, Pb e Zn) nos manguezais dos rios Aribiri, Bubu e Santa Maria da Vitória – Grande Vitória/ES – utilizando Crassotrea rhizophorae (Guildins, 1828) e Mytella guyanensis (Lamarck, 1819) como biomonitores. Dissertação de Mestrado. Vitória: Universidade Federal do Espírito Santo.
Svendsen C, Weeks JM (1995) The use of a lysosome assay for the rapid assessment of cellular stress from copper to the freshwater snail Viviparus contectus (Millet). Marine Pollution Bulletin 31: 139-142.
Vasconcellos MAF (2004) Bioacumulação e depuração de hidrocarbonetos poilicíclicos aromáticos no mexilhão Perna perna em ensaios de microcosmos. Dissertação de Mestrado em Engenharia Ambiental. Vitória: Universidade Federal do Espírito Santo.
How to Cite
License
Esta licença permite que outros distribuam, remixem, adaptem e criem a partir do seu trabalho, mesmo para fins comerciais, desde que lhe atribuam o devido crédito pela criação original.
