In vitro inhibition of mycelial growth of fungus Fusarium oxysporum f. sp. passiflorae using actinomycetes isolates obtained from rhizosphere of native plants from Bahian Cerrado

Abstract

This work aimed to study the in vitro antibiosis of actinomycetes obtained from the rhizosphere of plants of the cerrado of Bahia, on the fungus F. oxysporum f. sp. passiflorae evaluating their potential antagonism, aiming to support research in the field. For studies of in vitro antagonism of actinomycetes, the experiment was performed in DIC, using the method of paired cultures in petri dishes containing BDA medium. The treatments consisted of 6 actinomycete (Ac.M, Ac.J, Ac.R, Ac.O, Ac.B, Ac.P) compared to F. oxysporum f. sp. passiflorae and control with only the F. oxysporum f. sp. passiflorae being evaluated mycelial growth. At three days after the pairing of the colonies, only the actinomycete Ac.P not statistically different from the control. At seven and eleven days after pairing every actinomycete isolates tested in this study (Ac.M, Ac.J, Ac.R, Ac.O, Ac.B, Ac.P), were effective in inhibiting the mycelial growth Fusarium oxysporum f. sp. passiflorae able to laboratório. O percentage of inhibition varied with the type of isolated antagonistic used. And, among the actinomycetes tested statistical difference only between isolates Ac.R, which showed the highest percentage inhibition (35.85%) and isolated actinomycete Ac.J, in which we observed a lower percentage of inhibition (27.59%).